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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
23/09/2019 |
Actualizado : |
23/09/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
COUTO, D.; STRANSFELD, L.; ARRUABARRENA, A.; ZIPFEL, C.; LOZANO-DURÁN, R. |
Afiliación : |
DANIEL COUTO, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; LENA STRANSFELD, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CYRIL ZIPFEL, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; ROSA LOZANO-DURÁN, Sainsbury Laboratory, Norwich Research Park, United Kingdom.; Shanghai Institutes of Biological Sciences, Shanghai Center for Plant Stress Biology (PSC), Chinese Academy of Sciences, China. |
Título : |
Broad application of a simple and affordable protocol for isolating plant RNA. (Short Report). |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
BMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS. |
ISSN : |
1756-0500 |
DOI : |
10.1186/s13104-015-1119-7 |
Idioma : |
Inglés |
Notas : |
Article history: Published on: 16 April 2015. |
Contenido : |
Abstract View references (4)
Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide.
© 2015 Couto et al.; licensee BioMed Central. |
Palabras claves : |
Affordable; Arabidopsis; Protocol; RNA; Tomato; Wheat. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13321/1/Couto-et-al.-2015.-BMC-Research-Notes.pdf
https://bmcresnotes.biomedcentral.com/track/pdf/10.1186/s13104-015-1119-7
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Marc : |
LEADER 02324naa a2200277 a 4500 001 1060208 005 2019-09-23 008 2015 bl uuuu u00u1 u #d 022 $a1756-0500 024 7 $a10.1186/s13104-015-1119-7$2DOI 100 1 $aCOUTO, D. 245 $aBroad application of a simple and affordable protocol for isolating plant RNA. (Short Report).$h[electronic resource] 260 $c2015 500 $aArticle history: Published on: 16 April 2015. 520 $aAbstract View references (4) Background: Standard molecular biological methods involve the analysis of gene expression in living organisms under diverse environmental and developmental conditions. One of the most direct approaches to quantify gene expression is the isolation of RNA. Most techniques used to quantify gene expression require the isolation of RNA, usually from a large number of samples. While most published protocols, including those for commercial reagents, are either labour intensive, use hazardous chemicals and/or are costly, a previously published protocol for RNA isolation in Arabidopsis thaliana yields high amounts of good quality RNA in a simple, safe and inexpensive manner. Findings: We have tested this protocol in tomato and wheat leaves, as well as in Arabidopsis leaves, and compared the resulting RNA to that obtained using a commercial phenol-based reagent. Our results demonstrate that this protocol is applicable to other plant species, including monocots, and offers yield and purity at least comparable to those provided by commercial phenol-based reagents. Conclusions: Here, we show that this previously published RNA isolation protocol can be easily extended to other plant species without further modification. Due to its simplicity and the use of inexpensive reagents, this protocol is accessible and affordable and can be easily implemented to work on different plant species in laboratories worldwide. © 2015 Couto et al.; licensee BioMed Central. 653 $aAffordable 653 $aArabidopsis 653 $aProtocol 653 $aRNA 653 $aTomato 653 $aWheat 700 1 $aSTRANSFELD, L. 700 1 $aARRUABARRENA, A. 700 1 $aZIPFEL, C. 700 1 $aLOZANO-DURÁN, R. 773 $tBMC Research Notes, 16 April 2015, Volume 8, Issue 1, Article number 154. OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
14/08/2019 |
Tipo de producción científica : |
Actividades de Difusión |
Autor : |
INIA LA ESTANZUELA; BANCHERO, G.; FERNANDEZ, M.; GANZÁBAL, A. |
Afiliación : |
ESTACIÓN EXPERIMENTAL "DR. ALBERTO BOERGER", INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA EUGENIA FERNANDEZ DIEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRES RICARDO GANZÁBAL PLANINICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Producción ovina intensiva. |
Complemento del título : |
DÍA DE CAMPO, 23 de setiembre, Colonia, 2005. |
Fecha de publicación : |
2005 |
Fuente / Imprenta : |
La Estanzuela, Colonia (Uruguay): INIA, 2005. |
Páginas : |
19 p. |
Serie : |
(INIA Serie Actividades de Difusión ; 426) |
Idioma : |
Español |
Notas : |
Personal de Apoyo: Néstor Beltrán; Néstor Sanguinetti; Damián González. |
Palabras claves : |
FINNISH LANDRACE; TASA OVULATORIA. |
Thesagro : |
CORDERO; OVINOS; PRODUCCION ANIMAL; RAZAS (ANIMALES). |
Asunto categoría : |
A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/446/1/112761070710163559.pdf
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Marc : |
LEADER 00706nam a2200241 a 4500 001 1023331 005 2019-08-14 008 2005 bl uuuu u00u1 u #d 100 1 $aINIA LA ESTANZUELA 245 $aProducción ovina intensiva. 260 $aLa Estanzuela, Colonia (Uruguay): INIA$c2005 300 $a19 p. 490 $a(INIA Serie Actividades de Difusión ; 426) 500 $aPersonal de Apoyo: Néstor Beltrán; Néstor Sanguinetti; Damián González. 650 $aCORDERO 650 $aOVINOS 650 $aPRODUCCION ANIMAL 650 $aRAZAS (ANIMALES) 653 $aFINNISH LANDRACE 653 $aTASA OVULATORIA 700 1 $aBANCHERO, G. 700 1 $aFERNANDEZ, M. 700 1 $aGANZÁBAL, A.
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INIA Tacuarembó (TBO) |
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